![]() ![]() (Support: Breast Cancer Research Foundation and Hormel Foundation.) 161-P However, when serum leptin was elevated without E, MT growth was enhanced independent of body weight indicating a complex relationship of leptin and breast cancer. Integration of these findings indicates that when elevated serum leptin was associated with supplemental E, MT development was not impacted. Serum leptin followed a similar pattern as fat pad weights with 0.3 GTG obese mice having a 7-fold increase and NON-OB/noE and NON-OB/E mice had 107% and 74% higher leptin levels than SAL mice. Despite similar body weights, fat pad weights for NON-OB/noE and NON-OB/E mice were increased by 27% and 26%, respectively, compared to SAL mice. Fat pad weights for 0.3 GTG obese mice were 3-fold heavier than SAL mice. In comparison, 12.5%, 20% and 0% incidence rates were detected for SAL/noE, NON-OB/E and OB/E groups. For 0.3 GTG mice, OB/noE had a 100% incidence of MTs and NON-OB-noE had a 57% incidence. No MTs were detected in GTG-obese or SAL mice, and one GTG-nonobese mouse had a MT. Serum leptin of GTG-obese mice averaged 47 ng/mL. GTG-obese mice were 50% heavier than SAL and GTG-nonobese mice. 0.5 GTG mice were divided into GTG-obese (7/16) and GTG-nonobese (9/16) based on body weight relative to SAL. 0.3 GTG mice received cells and either E (n = 20) or noE (n = 20) or were SAL with noE (n = 10). At 10 wks of age 0.5 GTG mice were implanted with E pellets and inoculated with 5 × 10 6 T47-D human breast cancer cells in matrigel into the left mammary fat pad. Therefore, remaining mice received 0.3 mg/kg GTG. This dose elicited a high mortality (probably due to ovariectomies). At 6 wks of age CD-I female nude mice (n = 30) received 0.5 mg/kg body weight of GTG by ip injection and 10 mice received saline (SAL). Here we evaluated the effect of GTG-induced obesity in a xenograft model of ER-positive breast cancer. We previously found that diet-induced obesity and higher serum leptin were associated with shortened tumor latency and more palpable MTs in a transgenic mouse model. Leptin, an adipokine elevated in obesity, is among several factors including estrogen (E) implicated in breast cancer development. ![]() Obesity is a risk factor for estrogen receptor (ER) positive breast cancer in postmenopausal women. KATAI NKHATA, AMITABHA RAY, SONER DOGAN, JOSEPH GRANDE, MARGOT CLEARY Austin, MN Rochester, MN Gold Thioglucose (GTG)-Induced Obesity and T47-D Mammary Tumor (MT) Development in the Presence or Absence of Estrogen in a Xenograft Model Poster Session I Selected posters from 160-P through 397-P. ![]() Communications Assistant, The Orange Bowl Committee - Miami Lakes, FL JULY 2016 - JANUARY 2017.Saturday October 21 Viewing: 12:45 PM - 6:30 PM Presenters at Posters: 5:00 PM - 6:30 PM
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